Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro-derived plantlets of Bambusa nutans Wall., using AFLP markers

Bambusa nutans Wall., is an evergreen, perennial, and multipurpose bamboo having strong culms, which are largely used for construction, scaffolding, craft purposes, pulp, and paper industry

Rupali Mehta; Vikas Sharma; Anil Sood; Madhu Sharma; Ram Kumar Sharma

2010

Scholarcy highlights

  • Bambusa nutans Wall., is an evergreen, perennial, and multipurpose bamboo having strong culms, which are largely used for construction, scaffolding, craft purposes, pulp, and paper industry
  • Maximum shoots were achieved on MS medium fortified with BAP
  • Maturation and germination of well-organized somatic embryos was achieved on MS medium containing BAP and 2,4-D with 20.0 mg l−1 ascorbic acid
  • Four hundred and seven scorable fragments were amplified, of which 402 have recorded conservation at various morphogenetic stages leading to plantlets regeneration, revealed a high level of genetic stability
  • Full-strength MS medium supplemented with 2% glucose favored further development of proliferated somatic embryos into plantlets

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