Distribution of macrophages during fish development: an immunohistochemical study in carp ( Cyprinus carpio , L.)

A monoclonal antibody against carp macrophages has been utilised in flow cytometry, immuno-histochemistry and immuno-electron microscopy to assess the distribution of monocytes/macrophages in developing carp lymphoid tissues

N. Romano; S. Picchietti; J. J. Taverne-Thiele; N. Taverne; L. Abelli; L. Mastrolia; B. M. L. Verburg-van Kemenade; J. H. W. M. Rombout

2002

Scholarcy highlights

  • A monoclonal antibody against carp macrophages has been utilised in flow cytometry, immuno-histochemistry and immuno-electron microscopy to assess the distribution of monocytes/macrophages in developing carp lymphoid tissues
  • In suspensions of living cells WCL15 reacted strongly with cytoplasm and plasmic membrane of macrophages. It cross-reacted with a subpopulation of thrombocytes, but this reaction could be neglected by double immunostaining in combination with a thrombocyte-specific marker
  • Macrophages were found from day 2 post-fertilisation in head kidney and in the dorsal portion of the yolk sac epithelium
  • From 1 week onwards macrophages were found scattered in thymus and gut and during the second week in spleen
  • Macrophages increased in number in all lymphoid tissues until the 6–8th week post-fertilisation, but they decreased except in thymus, where they became localised mainly in the cortical-medullary boundary, and in white pulp areas of head kidney
  • The role of macrophages in allowing an early non-specific defence in young fish and in co-operating during the differentiation processes of T-cells and B-cells is discussed

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