Characterizations and fine mapping of a mutant gene for high tillering and dwarf in rice (Oryza sativa L.)

We show that the htd-1 mutant increases its tiller number by releasing axillary buds from dormant stage rather than by initiating more axillary buds

Junhuang Zou; Zengxiang Chen; Shuying Zhang; Weiping Zhang; Guanghuai Jiang; Xianfeng Zhao; Wenxue Zhai; Xuebiao Pan; Lihuang Zhu

2005

Scholarcy highlights

  • A rice htd-1 mutant, related to tillering and dwarfing, was characterized
  • We show that the htd-1 mutant increases its tiller number by releasing axillary buds from dormant stage rather than by initiating more axillary buds
  • The dwarf is caused by averagely reducing each internode and panicle. Based on this dwarfing pattern, the htd-1 mutant could be grouped into dn-type dwarf defined by Takeda
  • Based on the quantitative determination of IAA and ABA and application of the two hormones exogenously to the seedlings, we inferred that the high tillering capacity of the htd-1 mutant should not be attributed to a defect in the synthesis of IAA or ABA
  • Sequencing the target DNA region and comparing the counterpart DNA sequences between the htd-1 mutant and other rice varieties revealed a nucleotide substitution corresponding to an amino acid substitution from prolin to leucine in a predicted rice gene, OsCCD7, the rice orthologous gene of AtMAX3/CCD7
  • With the evidence of the association between the presence of one amino acid change in OsCCD7 and the abnormal phenotypes of the htd-1 mutant, OsCCD7 was identified as the candidate of the HTD1 gene

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