GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene

Two key fatty acid biosynthetic genes, stearoyl-ACP desaturase, and acylcarrier protein in Elaeis guineensis showed high level of expression during the period of oil synthesis in the mesocarp and kernel. Both genes are expressed in spear leaves at much lower levels and the expression increased by 1.5-fold to 2.5-fold following treatments with ethylene and abscisic acid

Farah Hanan Abu Hanifiah; Siti Nor Akmar Abdullah; Ashida Othman; Noor Azmi Shaharuddin; Halimi Mohd Saud; Hasnul Abdul Hakim Hasnulhadi; Umaiyal Munusamy

2018

Scholarcy highlights

  • Two key fatty acid biosynthetic genes, stearoyl-ACP desaturase, and acylcarrier protein in Elaeis guineensis showed high level of expression during the period of oil synthesis in the mesocarp and kernel. Both genes are expressed in spear leaves at much lower levels and the expression increased by 1.5-fold to 2.5-fold following treatments with ethylene and abscisic acid
  • The highest SAD1 promoter activity was observed in the mesocarp followed by kernel and the least in the leaves
  • It was found that the 49-bp region binds to the nuclear protein extract from mesocarp but not from leaves in electrophoretic mobility shift assay
  • Further fine-tuned analysis of this 49-bp region using truncated DNA led to the identification of GCTTCA as a novel motif in the SAD1 promoter
  • Keyword: Stearoyl-ACP desaturase; Acyl-carrier protein; Oil palm; Fatty acid biosynthesis; Promoter deletion analysis; TAAAAT motif

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