Toward rational control of Escherichia coli O157:H7 by a phage cocktail

Twenty six phages infected with Escherichia coli O157:H7 were screened from various sources

Y. Tanji; T. Shimada; M. Yoichi; K. Miyanaga; K. Hori; H. Unno

2004

Scholarcy highlights

  • Twenty six phages infected with Escherichia coli O157:H7 were screened from various sources
  • Prolonged incubation of E. coli cells and phage allowed the emergence of phage-resistant cells
  • A rational procedure for selecting an effective cocktail of phage for controlling bacteria was investigated based on the mechanism of phage-resistant cell conversion
  • Deletion of OmpC from the E. coli cells facilitated the emergence of cells resistant to SP21 phage
  • When a cocktail of phages SP21 and SP22 was used to infect E. coli O157:H7 cells, 30 h was required for the emergence of cells resistant to both phages
  • Even though required for the emergence of cells cells resistant to both phages appeared, this work shows that rational selection of phages has the potential to at least delay the emergence of phage resistance

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