Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro

Our results showed that in order to estimate cell viability after cell treatment with combination of the bleomycin and electroporation the most reliable method is clonogenic assay

Baltramiejus Jakštys

2015

Scholarcy highlights

  • The aim of this study was to compare different and commonly used cell viability assays after CHO cells treatment with anticancer drug bleomycin, high voltage electric pulses, and combination of bleomycin and HV electric pulses
  • MTT assay performed at 6–72 h time points after these treatments revealed that MTT cell viability is highly dependent on evaluation time point and decreased with later evaluation time points
  • In comparison to clonogenic cell viability, MTT cell viability after bleomycin electrotransfer at all testing time points was significantly higher
  • Flow cytometry assay if used at later times, 2–3 days after the treatment, allowed reliable evaluation of cell viability
  • Our results showed that in order to estimate cell viability after cell treatment with combination of the bleomycin and electroporation the most reliable method is clonogenic assay
  • Improper use of propidium iodide and MTT assays can lead to misinterpretation of the experimental results
  • Previous research Electroporation enhances mitomycin C cytotoxicity on T24 bladder cancer cell line: a potential improvement of intravesical chemotherapy in bladder cancer

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