Mining and characterizing microsatellites from citrus ESTs

Available computer programs were arranged in a pipeline to extract microsatellites from public citrus EST sequences, retrieved from the NCBI

Chunxian Chen

2006

Scholarcy highlights

  • Available computer programs were arranged in a pipeline to extract microsatellites from public citrus EST sequences, retrieved from the NCBI
  • 3,278 bi- to hexa-type SSR-containing sequences were identified from 56,199 citrus ESTs
  • One SSR was found per 5.2 kb of EST sequence, with the tri-nucleotide motifs as the most abundant
  • Primer sequences flanking SSR motifs were successfully identified from 2,295 citrus ESTs
  • A subset were synthesized and tested to determine polymorphism and heterozygosity between/within two genera, sweet orange and Poncirus, which are the parents of the citrus core mapping population selected for an international citrus genomics effort
  • These SSR primer sequences are available upon request

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