Hematopoietic Capability of CD34+ Cord Blood Cells: A Comparison With CD34+ Adult Bone Marrow Cells

These results demonstrated that human hematopoiesis changes with development from fetus to adult

Takahiro Ueda

2008

Scholarcy highlights

  • The characteristics of hematopoietic progenitor and stem cell populations in mammals vary according to their ontogenic stage
  • We compared CD34+ cells, a hematopoietic cell population, in cord blood with those in adult bone marrow using phenotypic subpopulations analyzed by flow cytometry, the colony-forming activity in methylcellulose clonal cultures, and the repopulating ability of these cells in NOD/Shi-scid mice
  • The proportion of CD34+ cells was higher in adult BM than in CB mononuclear cells, the more immature subpopulations, CD34+CD33- and CD34+CD38- cells, were present in higher proportions in CD34+ CB cells
  • Clonal culture assay showed that more multipotential progenitors were present in CD34+ CB cells
  • When transplanted into NOD/SCID mice, CD34+ adult BM cells could not reconstitute human hematopoiesis in recipient BM, but CD34+ CB cells achieved a high level of engraftment, indicating that CD34+ CB cells possess a greater repopulating ability
  • CD34+ CB cells contained a greater number of primitive hematopoietic cells, including HSCs, than did adult BM, suggesting the usefulness of CD34+ CB cells as a graft for therapeutic HSC transplantation and as a target cell population for ex vivo expansion of transplantable HSCs and for gene transfer in gene therapy

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