A simple, rapid, specific and sensitive method for the detection of serum hepatitis C virus RNA using the reverse transcription-polymerase chain reaction technique without conventional RNA extraction was developed
2008
Key concepts
Scholarcy highlights
- A simple, rapid, specific and sensitive method for the detection of serum hepatitis C virus RNA using the reverse transcription-polymerase chain reaction technique without conventional RNA extraction was developed
- HCV template RNA from serum was obtained by boiling the serum at 95°C for 2 min, cooling rapidly in ice and removing the proteins by cetrifugation
- RT-PCR amplifications including the reverse transcription and first PCR amplification were performed in one vessel containing both of reverse transcriptase andTaq DNA polymerase
- The detection of HCV RNA from 10−3 μl serum was possible with this method
- Detections of HCV RNA with this method were in 4 of 13 anti-HCV antibody negative samples and 95 of 212 positive samples
- H., Okada, S., Sugiyama, Y., Tanaka, T., Sugai, Y., Akahan, Y., Machida, A., Mishiro, S., Yoshizawa, H., Miyakawa, Y. and Mayumi, M., Detection of hepatitis C virus by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5′-noncoding region.Jpn
- Rapid detection of serum hepatitis C virus RNA by combining reverse transcription and polymerase chain reaction without RNA extraction
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