A simple and efficient method for DNA extraction from grapevine cultivars andVitis species

A quick, simple, and reliable method for the extraction of DNA from grapevine species, hybrids, andAmpelopsis brevipedunculata has been developed. This method, based on that of Doyle and Doyle, is a CTBA-based extraction procedure modified by the use of NaCl to remove polysaccharides and PVP to eliminate polyphenols during DNA purification

Muhammad A. Lodhi

2008

Scholarcy highlights

  • A quick, simple, and reliable method for the extraction of DNA from grapevine species, hybrids, andAmpelopsis brevipedunculata has been developed. This method, based on that of Doyle and Doyle, is a CTBA-based extraction procedure modified by the use of NaCl to remove polysaccharides and PVP to eliminate polyphenols during DNA purification
  • DNA is completely digestible with restriction endonucleases and amplifiable in the polymerase chain reaction, indicating freedom from common contaminating compounds
  • An effective method of DNA isolation from the mature leaves ofGossypium species that contain large amounts of phenolic terpenoids and tannins

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