A simple and efficient method for DNA extraction from grapevine cultivars andVitis species

Muhammad A. Lodhi


A quick, simple, and reliable method for the extraction of DNA from grapevine species, hybrids, andAmpelopsis brevipedunculata has been developed. This method, based on that of Doyle and Doyle, is a CTBA-based extraction procedure modified by the use of NaCl to remove polysaccharides and PVP to eliminate polyphenols during DNA purification


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