Oxygen protection of nitrogenase in Frankia sp. HFPArI3

O2 protection of nitrogenase in a cultured Frankia isolate from Alnus rubra was studied in vivo

Marcia A. Murry

2004

Key concepts

Scholarcy highlights

  • O2 protection of nitrogenase in a cultured Frankia isolate from Alnus rubra was studied in vivo
  • Evidence for a passive gas diffusion barrier in the vesicles was obtained by kinetic analysis of in vivo O2 uptake and acetylene reduction rates in response to substrate concentration
  • The apparent Km for acetylene was more than 10-fold greater than reported in vitro values. These data were inter oreted as evidence for a gas diffusion barrier in the vesicles but not vegetative filaments of Frankia sp

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