Calorimetric and EPR studies of the thermotropic phase behavior of phospholipid membranes

Transmission electron micrographs showed that liposome vesicles prepared from DL-α-phosphatidylcholine dimyristoyl by the modified reverse-phase evaporation method were spherical in shape and in majority of them were less than 100 nm in diameter

Danuta Pentak

2008

Scholarcy highlights

  • Transmission electron micrographs showed that liposome vesicles prepared from DL-α-phosphatidylcholine dimyristoyl by the modified reverse-phase evaporation method were spherical in shape and in majority of them were less than 100 nm in diameter
  • Differential scanning calorimetry method was used to determine the influence of cholesterol content and pH of Tris-HCl buffer used for the preparation of liposomes on the temperature of phase transition TC of phospholipids which form the investigated liposome vesicles
  • TEMPO can be used to observe the change in partition between aqueous and fluid lipid regions
  • The change in the relative values of f determined for DMPC as a function of temperature shows that this phospholipid undergoes a transition from a ‘gel phase’ to a lamellar smectic liquid crystalline phase in the presence of excess water
  • The results were compared with those obtained with Differential scanning calorimetry method

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