A light and electron microscopic study of the intraneuronal transport of horseradish peroxidase and wheat germ agglutinin-peroxidase conjugates in the rat visual system

The ability of horseradish peroxidase and the lectin wheat germ agglutinin covalently conjugated with HRP to label retrogradely dorsal lateral geniculate nucleus neurons, subsequent to injections of either marker into rat striate cortex, was assessed by counting labelled neurons in the dLGN

John Q. Trojanowski

2005

Scholarcy highlights

  • The ability of horseradish peroxidase and the lectin wheat germ agglutinin covalently conjugated with HRP to label retrogradely dorsal lateral geniculate nucleus neurons, subsequent to injections of either marker into rat striate cortex, was assessed by counting labelled neurons in the dLGN
  • In additional rats, which received striate cortex injections of 100μg/μ1 of either free HRP or HRP coupled to WGA, the injection site was studied with electron microscopy after survivals of 30 min to 24 h
  • Similar organelles in neuronal perikarya, dendrites and axons were labelled by both markers, with the exception that only rats injected with WGA-HRP had labelled GERL in some of their neurons in striate cortex
  • Ultrastructural correlates by a new technique.Journal of Histochemistry and Cytochemistry14, 291–302

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