Effect of oxygen on substrate utilization for nitrogen fixation and growth in Frankia spp.

These results suggested that trehalose was oxidized in the vesicles as well as in the vegetative hyphae

Mary F. Lopez

2004

Key concepts

Scholarcy highlights

  • Frankia, the actinomycete partner in the nitrogenfixing symbiosis of certain woody non-legumes, has been shown to fix nitrogen in pure culture under aerobic conditions
  • The carbon source utilized had an effect on the optimum O2 concentration for acetylene reduction
  • Oxygen uptake kinetics showed that respiration in vesicle-containing cells utilizing trehalose had a biphasic response to oxygen concentration with a diffusion limited component at oxygen concentrations of 20 μM to more than 300 μM
  • These results suggested that trehalose was oxidized in the vesicles as well as in the vegetative hyphae
  • Cells grown with 5–10% O2 in the gas phase had a doubling time approximately half those grown with 20% O2
  • Trehalose supported approximately 40% lower rates of oxygen uptake than propionate in vesicle-containing cells

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