Plant viral leaders influence expression of a reporter gene in tobacco

In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector

Mandy J. Dowson Day

2004

Scholarcy highlights

  • In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector
  • In transgenic tobacco the improved GUS levels were maintained from constructs including either the tobacco mosaic virus leader or this sequence with the addition of the 35S transcription initiation site bases
  • A comparison of GUS enzyme amounts with GUS mRNA amounts, using the chloramphenicol acetyl transferase gene as an internal standard, revealed that TMV leader-bearing mRNA was translated from four-to six-fold more efficiently than the random leader control
  • The 5′ leader of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo
  • The 5′-untranslated leader sequence of potato virus X RNA enhances the expression of a heterologous gene in vivo
  • Dowson Day, M.J., Ashurst, J.A., Mathias, S.F. et al Plant viral leaders influence expression of a reporter gene in tobacco

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