Automated monitoring of cell concentration and viability using an image analysis system

In order to automate measurements of cell concentration and viability in a suspended animal cell culture, we have developed an in situ microscopic image analysis system with an effective cell recognition algorithm

Fumio Maruhashi

2013

Scholarcy highlights

  • In order to automate measurements of cell concentration and viability in a suspended animal cell culture, we have developed an in situ microscopic image analysis system with an effective cell recognition algorithm
  • With a small amount of sample, this system can measure the cell density rapidly and aseptically. It can measure a cell size histogram including cell debris small particle distribution. These small particles have been found to be related to the viability of the mouse-mouse hybridoma STK1 cell line
  • Chuck AS & Palsson BO Population Balance between Producing and Nonproducing Hybridoma Clones Is very Sensitive to Serum Level, State of Inoculum, and Medium Composition

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