Analytical Applications of UV-VIS Spectroscopy

For about 130 years, the Bouguer-Lambert-Beer law has been used as the quantitative basis of absorption spectroscopy

Heinz-Helmut Perkampus

2012

Scholarcy highlights

  • For about 130 years, the Bouguer-Lambert-Beer law has been used as the quantitative basis of absorption spectroscopy
  • Bouguer established empirically a correlation between pathlength and light absorption in 1729. This correlation was formulated mathematically by Lambert in 1760 and Beer discovered the dependence upon the concentration in 1852
  • The human eye was the detector for comparing different light intensities
  • In 1925, Pulfrich introduced his photometer as “a photometer appropriately adapted to exceed the levels of sensitivity of the human eye, called a step photometer…”
  • Our eyes are capable of assessing the uniformity of two light densities with an accuracy of approximately 1%
  • The principle of colorimetry or visual photometry is based on this fact

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