Detection of Crimean–Congo Hemorrhagic Fever, Hanta, and Sandfly Fever Viruses by Real-Time RT-PCR

This chapter describes four fluorescence-based PCR assays to detect the Crimean–Congo Hemorrhagic Fever, Andes, Hantaan, and Sandfly Fever Sicilian Viruses. These assays are based on species-specific hydrolysis probes targeting the nucleocapsid protein gene for CCHFV and SFSV and the glycoprotein gene for ANDV and HANV

Sofi M. Ibrahim; Mohamed Aitichou; Justin Hardick; Jamie Blow; Monica L. O’Guinn; Connie Schmaljohn

2010

Scholarcy highlights

  • This chapter describes four fluorescence-based PCR assays to detect the Crimean–Congo Hemorrhagic Fever, Andes, Hantaan, and Sandfly Fever Sicilian Viruses. These assays are based on species-specific hydrolysis probes targeting the nucleocapsid protein gene for CCHFV and SFSV and the glycoprotein gene for ANDV and HANV
  • The sensitivity and specificity of the assays were evaluated with test panels that consisted of 20–60 known positive and 30–135 known negative samples, representing 7–34 genetically diverse bacterial and viral species
  • The United States Army Medical Research Institute of Infectious Diseases, Frederick, MD, USA

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