This chapter describes four fluorescence-based PCR assays to detect the Crimean–Congo Hemorrhagic Fever, Andes, Hantaan, and Sandfly Fever Sicilian Viruses. These assays are based on species-specific hydrolysis probes targeting the nucleocapsid protein gene for CCHFV and SFSV and the glycoprotein gene for ANDV and HANV
2010