In situ Hybridization Histochemistry as a Tool for the Study of Brain Function

Nojiri, H., Sato, M. and Urano, A.

Frank Baldino

2015

Scholarcy highlights

  • Nojiri, H., Sato, M. and Urano, A.
  • In situ hybridization histochemistry has become a powerful tool for the study of specific peptide or protein biosynthesis at the level of the single neuron
  • This technique has frequently been used to confirm immunoreactive neurons as the actual site of biosynthesis, the greatest utility is apparent in studies of altered gene expression in development or pathology, or due to physiological or pharmacological manipulations
  • The goal of this chapter is to provide the reader with essential background information for understanding the technology of in situ hybridization histochemistry and describe some of its applications, to help the reader to appreciate the potential utility of this technology for the analysis of brain function and its disorders
  • In situ hybridization of the vasopressin mRNA in the rat hypothalamus by use of a synthetic oligonucleotide probe

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