Protein Microchips: Use for Immunoassay and Enzymatic Reactions

To confirm a possible application of the enzyme microchips for purposes of combinatorial analysis and drug discovery, we studied kinetics of enzymatic reaction and inhibition on a microchip

Pavel Arenkov

2003

Scholarcy highlights

  • The development and application of the arrays of immobilized biological compounds have become a significant trend in modern biology, biotechnology, and medicine
  • The rather low porosity of conventional polyacrylamide gels retards diffusion into the gel of the macromolecules that interact with the immobilized compounds
  • Several types of immunoassays were performed on protein microchips in which either the antigen or the antibody was immobilized within the gel pads and detected directly with labeled antibodies or antigens, respectively
  • Microarrays of gel pads containing immobilized proteins were manufactured with the same technique developed earlier for making oligonucleotide and DNA microchips
  • The procedure consists of three steps: manufacturing the micromatrix of polyacrylamide gel pads by photopolymerization, application of a protein solution onto each gel pad by a pin, and, chemical fixation of the proteins within the gel pads
  • A two-step procedure was suggested for manufacturing oligonucleotide and protein gel-based microchips involving a copolymerization of acrylamide with these compounds
  • Antibody or antigen microchips were applied to detect complementary molecules in the fluorescence or enzymatic direct or indirect immunoassay

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