Differential expression of peroxiredoxins in prostate cancer: Consistent upregulation of PRDX3 and PRDX4

Because their significance in prostate cancer is unclear, we investigated their expression and clinical associations in PCa

Anamika Basu; Hiya Banerjee; Heather Rojas; Shannalee R. Martinez; Sourav Roy; Zhenyu Jia; Michael B. Lilly; Marino De León; Carlos A. Casiano


Scholarcy highlights

  • Prostate cancer is the most frequently diagnosed male cancer and the second leading cause of cancer death in men in the United States
  • Immunoblotting analysis of PRDX protein expression in a panel of 11 prostate cell lines revealed that most PRDXs exhibited elevated expression in transformed or tumor-derived cell lines, compared to their relatively low levels in the primary normal cell lines PrEC and PrSC, and the benign prostatic hyperplasia cell line BRF-55T
  • Differences in PRDX protein expression levels between tumor and non-tumor cell lines were most dramatic for PRDX1 and 3
  • The elevated levels of PRDX3 in the AA-derived cell line MDA-prostate cancer-2b, its robust overexpression in prostate tumors compared to normal tissues, and its association with increase in tumor stage and PSA, prompted us to analyze its expression in ethnicity PCa tissue microarrays containing 150 cases from AA PCa patients and 150 cases from CC PCa patients
  • In our study we found that AA patients with a Gleason grade 4+3 had a shorter PSA recurrence free survival compared to CC patients, which would be consistent with the notion that the AA patients had a more aggressive disease
  • We observed that high PRDX3 expression significantly correlated with elevated Gleason sum score in the total AA group, comparison of low and high PRDX3 expression with PRFS in AA patients showed no significant difference
  • It is possible that the CC group in the ethnicity TMA included more men in which PCa was detected at earlier tumor stage compared to the AA group, which could explain the more favorable disease outcome in CC patients with low PRDX3 expression
  • Their role in prostate cancer development, and their potential as biological determinants of PCa health disparities and novel therapeutic targets, deserve further investigation

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