On the mechanism of genetic transfer during conjugation ofEscherichia coli

(1) The DNA molecules transferred and recovered after mating from the recipient cells are synthesized during mating; one strand of these DNA molecules is a parental DNA strand which comes from the parental donor cell

Fran�ois Cuzin; G�rard Buttin; Fran�ois Jacob

2005

Scholarcy highlights

  • (1) The DNA molecules transferred and recovered after mating from the recipient cells are synthesized during mating; one strand of these DNA molecules is a parental DNA strand which comes from the parental donor cell
  • It seems likely that the transfer of genetic material observed upon mating of male strains of Escherichia coli K12 is due to a particular replication of the donor DNA
  • The detailed mechanism of genetic transfer is discussed in the light of different, recently published experimental results
  • These data do not lead to a clear and definite conclusion at the present time, some points are clearly established: The DNA molecules transferred and recovered after mating from the recipient cells are synthesized during mating; one strand of these DNA molecules is a parental DNA strand which comes from the parental donor cell
  • (3) One of the most interesting aspects of this problem is the identification of the mechanism which controls the transfer replication
  • The Hfr chromosome results from the fusion of two replicons, and an attractive hypothesis is that the chromosomal replicon controls the vegetative replication of the whole, and the sex factor controls transfer replication
  • This model has not yet been firmly demonstrated, it is supported by some arguments

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