Cytologic diagnosis of Ewing sarcoma/peripheral neuroectodermal tumor with paired prospective molecular genetic analysis

The current results demonstrated that molecular genetic techniques can provide clinically useful ancillary information for fine-needle aspiration specimens when cytologic features and/or immunophenotype are equivocal on the basis of limited sampling or secondary changes, such as hemorrhage and/or necrosis

Souzan Sanati

2007

Scholarcy highlights

  • Ewing sarcoma/peripheral neuroectodermal tumor, since its characterization immunophenotypically and cytogenetically, has emerged as one of most common sarcomas of childhood
  • We demonstrate that fine-needle aspiration biopsy material provides a sufficient substrate for molecular genetic testing by either fluorescence in situ hybridization or reverse transcriptase-polymerase chain reaction but that molecular analysis of cytology specimens shares many of the same limitations that impact the usefulness of this testing when applied to tissue specimens
  • A head computed tomography scan showed a 7-cm mass involving the parotid gland with effacement of the right parapharyngeal space and distortion of the nasopharynx; an abdominal CT scan showed multiple liver metastases
  • EWS/PNET traditionally has been considered a neoplasm of children and adolescents that presents in the soft tissue and bone
  • Previous reports have indicated that FNA biopsy is a reliable approach for the diagnosis of EWS/ PNET regardless of the site of clinical presentation
  • The so-called large cell variant of EWS/PNET may be confused with large cell lymphoma, but immunophenotyping settles this diagnostic issue in most cases
  • The current results demonstrate that ancillary testing of patients with EWS/PNET by using reverse transcriptase-polymerase chain reaction and/or fluorescence in situ hybridization is not limited by the lack of excisional biopsy material but can be performed on cytology specimens without compromising diagnosis by conventional cytologic and immunocytochemical analysis

Need more features? Save interactive summary cards to your Scholarcy Library.