Strigolactones: Plant Hormones with Promising Features

The use of fluorogenic molecules like yoshimulactone green that fluoresce upon hydrolysis, can proivde information about the chemical scaffolds of the synthetic molecules that efficiently compete with its binding as described for S. hermonthica HTL7. An in vitro yeast-two-hybrid assay has been developed to provide information on the strigolactone-promoted D14 interaction with MAX2/D3. StrigoQuant is a biological sensor that monitors strigolactone activity by measuring D14induced degradation of AtSMXL6 coupled to a luciferase in Arabidopsis protoplasts

Harro J. Bouwmeester; Raymonde Fonne‐Pfister; Claudio Screpanti; Alain De Mesmaeker

2019

Scholarcy highlights

  • Just as for the first plant hormone to be discovered, auxin, the development of synthetic analogs of strigolactones will be decisive to fully understand their biological relevance and explore applications in agriculture
  • The use of fluorogenic molecules like yoshimulactone green that fluoresce upon hydrolysis, can proivde information about the chemical scaffolds of the synthetic molecules that efficiently compete with its binding as described for S. hermonthica HTL7. An in vitro yeast-two-hybrid assay has been developed to provide information on the strigolactone-promoted D14 interaction with MAX2/D3. StrigoQuant is a biological sensor that monitors strigolactone activity by measuring D14induced degradation of AtSMXL6 coupled to a luciferase in Arabidopsis protoplasts
  • Arabidopsis expressing D14 fused to a luciferase can provide information about D14 degradation upon the application of strigolactones and provide quantitative activity information
  • The first strigolactone, strigol, was identified over 50 years ago and for many decades we did not know much more about the strigolactones than that plants produce several different variants and that these induce germination of seeds of root parasitic plants
  • Our greatly improved understanding of strigolactonesynthesis, perception and downstream signaling are key in an efficient development of these possibilities
  • More fundamental research on the roles of the strigolactones in and outside the plant will further fuel the development of strigolactones into the tools we will need to make modern-day agriculture more sustainable

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