Spectrophotometric Determination of Protein Concentration

This unit describes spectrophotometric and colorimetric methods for measuring the concentration of a sample protein in solution

Michael H. Simonian

2004

Scholarcy highlights

  • This unit describes spectrophotometric and colorimetric methods for measuring the concentration of a sample protein in solution
  • A spectrofluorometer or a filter fluorometer can be used to measure the intrinsic fluorescence emission of a sample solution; this value is compared with the emissions from standard solutions to determine the sample concentration
  • The fluorescence emission method is used to quantify purified protein. This simple method is useful for dilute protein samples and can be completed in a short amount of time
  • There are two colorimetric methods: the Bradford colorimetric method, based upon binding of the dye Coomassie brilliant blue to the protein of interest, and the Lowry method, which measures colorimetric reaction of tyrosyl residues in the protein sample

Need more features? Save interactive summary cards to your Scholarcy Library.